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Measuring nerve growth factor in saliva by immunoassay: A cautionary note

TitleMeasuring nerve growth factor in saliva by immunoassay: A cautionary note
Publication TypeJournal Article
Year of Publication2016
AuthorsMatin, MJ, Li, D, Peterson, J, Taylor, MK, Laurent, HK, Lucas, T, Granger, SJ, Granger, DA, Granger, SW
JournalPsychoneuroendocrinology
Volume63
Pagination235-7
Date PublishedJan
ISBN Number1873-3360 (Electronic)0306-4530 (Linking)
Accession Number26519777
KeywordsImmunoassay, Interference, Methods, Nerve growth factor, saliva
Abstract

Nerve growth factor (NGF), a neurotrophin, modulates a diverse set of physiologic processes in the nervous, immune, and endocrine systems. Studies suggest that NGF can be measured in saliva (sNGF). Historically, the method for measuring sNGF involves the off-label use of an enzyme immunoassay designed for use with cell-culture supernatants/tissue extracts (Nam et al., 2007; Ruhl et al., 2004). In a series of experiments we reveal this measurement strategy is subject to non-specific interference by constituents present in oral fluids. We conclude that the measurement of sNGF by this assay is not optimal for use with oral fluid specimens.